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KMID : 0043320040270121284
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Archives of Pharmacal Research
2004 Volume.27 No. 12 p.1284 ~ p.1289
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Galactosylated Chitosan (GC)-graft-Poly(vinyl pyrrolidone) (PVP) as Hepatocyte-Targeting DNA Carrier: In Vitro Transfection
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Park In-Kyu
Jiang Hu-Lin
Cook Seung-Eun
Cho Myung-Haing
Kim Su-Il
Jeong Hwan-Jeong
Akaike Toshihiro
Cho Chong-Su
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Abstract
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Galactosylated chitosan-graft-poly(vinyl pyrrolidone) (GCPVP) was synthesized and characterized for hepatocyte-targeting gene carrier. GCPVP itself as well as GCPVP/DNA complex had negligible cytotoxicity regardless of the concentration of GCPVP and the charge ratio, but GCPVP/DNA complex had slightly cytotoxic effect on HepG2 cells only in the case of the higher charge ratio and 20 mM of concentration used. Through the confocal laser scanning microscopy, it is shown that the endocytosis by interaction between galactose ligands of GCPVP and ASGPR of the hepatocytes was the major route of transfection of GCPVP/F-plasmid complexes.
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KEYWORD
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Galactosylated chitosan, Poly(vinyl pyrrolidone), Hepatocyte, Gene delivery
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